Intracellular localization and processing of Pseudomonas aeruginosa ExoS in eukaryotic cells
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چکیده
منابع مشابه
The intracellular localization of Pseudomonas aeruginosa lectins.
The localization of the Pseudomonas aeruginosa lectins (PA-I and PA-II) was studied using methods of osmotic shock, freezing and thawing and spheroplast formation. Very slight release of the two lectins occurred when P. aeruginosa was exposed to magnesium-osmotic shock or was frozen and thawed. Under these conditions, release of the periplasmic 5'-nucleotidase occurred, whereas no release of cy...
متن کاملMembrane localization contributes to the in vivo ADP-ribosylation of Ras by Pseudomonas aeruginosa ExoS.
Type III-delivered exoenzyme S (ExoS) preferentially ADP-ribosylated membrane-associated His(6)HRas, relative to its cytosolic derivative His(6)HRas Delta CAAX. This indicates that the subcellular protein distribution contributes to in vivo ADP-ribosylation by ExoS.
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Neutrophils are the first line of defense against bacterial infections, and the generation of reactive oxygen species is a key part of their arsenal. Pathogens use detoxification systems to avoid the bactericidal effects of reactive oxygen species. Here we demonstrate that the Gram-negative pathogen Pseudomonas aeruginosa is susceptible to reactive oxygen species but actively blocks the reactiv...
متن کاملExpression of Pseudomonas aeruginosa toxin ExoS effectively induces apoptosis in host cells.
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that primarily infects immunocompromised individuals and patients with cystic fibrosis. Invasive strains of P. aeruginosa are known to induce apoptosis at a high frequency in HeLa cells and in many other cell lines, a process that is dependent on the ADP-ribosylation (ADPRT) activity of a type III secreted protein ExoS. In our previo...
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Berk, Richard S. (Wayne State University, Detroit, Mich.). Intracellular hemolysin of Pseudomonas aeruginosa. J. Bacteriol. 85:522-526. 1963.-Disruption of cells (2 to 6 days old) of Pseudomonas aeruginosa grown in Tryptose Broth devoid of blood yielded an intracellular hemolysin active on sheep and human erythrocytes. The intracellular agent was not usually detectable in cells grown under high...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2000
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2000.01990.x